Course Summary

Students will review basic principles of light and electron microscopy and learn a variety of advanced microscopy methods through 1 lecture hour per week and 3-4 hours of hands-on training. Students will also be able to sign up for additional instrument time to practice their skills with a technician available to answer questions. Topics to be covered in lectures will be: Introduction to optics; Principles of light and electron microscopy; Sample preparation for light and electron microscopy; Freeze-fracture electron microscopy; Scanning probe microscopies; Cryoelectron microscopy; Confocal microscopy; Live cell methods; Immunocytochemistry; In-situ hybridization; High voltage EM; Computer-aided microscopy; Image processing, analysis and 3-D Visualization.

 

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