Research Description: 

Dr. Wang’s research includes the development of genetically-encoded molecule biosensors based on fluorescence resonance energy transfer (FRET) and the application of these biosensors for the visualization and quantification of molecular signals in live cells with high spatiotemporal resolution under physical/mechanical environment. The other interest in the lab is to develop molecular machines which can reprogram the cellular functions.

Graduate Program: 
2013 60. Sun J., Lu S., Ouyang M., Lin L.J., Zhuo Y., Liu B., Chan R., Chien S., Neel B.G., Wang Y. (2013) A Circuit Algorithm of Tunable Intramolecular Interactions Embedded within Shp2, Nature Communications, Accepted 59. Lu S., Huang H., Wang Y., Strongin AY., Wang Y. (2013) Highly Sensitive FRET Biosensors for Detecting MMP Activity in Live Breast Cancer Cells, PLoS ONE,Accepted 58. Seong J., Wang N., Wang Y. (2013) Mechanotransduction at Focal Adhesions: from Physiology to Cancer Development, Journal of Cellular and Molecular Medicine, Accepted 57. Ouyang M., Lu S., Chen CN., Kim T., Seong J., Leckband DE., Wang F., Reynolds AB., Schwartz MA., Wang Y. (2013) N-cadherin Differentially Couples with p120ctn and b-catenin to Regulate the Polarity of Distinct Molecular Activities, Nature Communications, Accepted 56. Lin L.-J., Grimme J.M., Sun J., Lu S., Gai L., Cropek D.M., Wang Y. (2013) The antagonistic roles of PDGF and integrin αvβ3 in regulating ROS production at focal adhesions, Biomaterials, Accepted 2012 55. Qian T., Lu S., Ma H., Fang J., Zhong W., Wang Y. (2012) FRET Imaging of Microenvironment Effects on Calcium Signaling in Live Cells, Integrative Biology, Accepted 54. Wang R., Lei L., Wang Y., Levine A.J., and Popescu G. (2012) Dispersion-relation fluorescence spectroscopy, Physics Review Letter, Accepted 53. *Liao X., *Lu S., Zhuo Y., Wang Y. (2012) Visualization of Src and FAK activity during the differentiation process from HMSCs to osteoblast by FRET biosensor, PLoS ONE, Accepted 52. Eichorst JP., Clegg RM., Wang Y. (2012), Red-Shifted Fluorescent Proteins Monitor Enzymatic Activity in Live HT-1080 Cells with Fluorescence Lifetime Imaging Microscopy (FLIM), Journal of Microscopy, Accepted 51. Birukova A.A., Tian Y., Dubrovskyi O., Zebda N., Sarich N., Tian X., Wang Y., Birukov K.G. (2012) VE-cadherin Trans-interactions Modulate Rac Activation and Enhancement of Lung Endothelial Barrier by Iloprost, Journal of Cellular Physiology, Accepted 50. Ouyang M., Lu S., Wang Y. (2012) Genetically Encoded Fluorescent Biosensors for Live-cell Imaging of MT1-MMP Protease Activity, Methods in Molecular Biology, In Press 2011 49. *Xiang X., *Sun J., Wu J., He H.T., Wang Y., Zhu C. (2011) A FRET-based biosensor for imaging SYK activities in living cells, Cellular and Molecular Bioengineering, Accepted 48. Nishitani WS., Saif TA, Wang Y. (2011) Calcium signaling in live cells on elastic gels under mechanical vibration at subcellular levels, PLoS ONE, Accepted 47. *Liao X., *Lu S., Zhuo Y., Winter C., Xu W., Li B., Wang Y. (2011) Bone physiology, biomaterial and the Effect of mechanical/physical microenvironment onmesenchymal stem cell osteogenesis, Cellular and Molecular Bioengineering, Accepted 46. Seong J., Ouyang M., Kim T., Sun J., Wen P.-C., Lu S., Zhuo Y., Llewellyn N.M., Schlaepfer D.D., Guan J.L., Chien S., Wang Y. (2011) Detection of focal adhesion kinase activation at Membrane Microdomains by fluorescence resonance energy transfer, Nature Communications, Accepted 45. Lu S., Kim T., Chen CN., Ouyang M., Seong J., Wang Y. (2011) Computational Analysis of the Spatiotemporal Coordination of Molecular Activities in Live Cells, PLoS ONE, 6(6):e21293 44. Eichorst JP., Huang H., Clegg RM., Wang Y. (2011) Phase differential enhancement to distinguish fluorescence components when monitoring molecular activity in live cells by FLIM, Journal of Fluorescence, 21(4):1763-77 43. Liu B., Lu S., Zheng S., Jiang Z.-L., Wang Y. (2011) Two Distinct Phases of Calcium Signaling under Flow, Cardiovascular Research, 91(1):124-33 42. Seong J., Lu S., Wang, Y. (2011) Live Cell Imaging of Molecular Signaling by FRET, Cellular and Molecular Bioengineering, 4(2):138-147 41. Butler P.J., Wang, Y. (2011) Editorial Note: Molecular Imaging and Mechanobiology, Cellular and Molecular Bioengineering, 4(2):123-124 40. Asada HH., Wang Y., Mayalu MN. (2011) Molecular Signaling Observer and Predictor: A Framework for Closed-Loop Control of Cell Behaviors Having Long Time Delay, American Control Conference. 1240:2314-2319 2010 39. Lu, S., Wang, Y. (2010) FRET Biosensors for Cancer Detection and Evaluation of Drug Efficacy, Clinical Cancer Research, 16(15):3822-4 38. Gawlik K., Remacle AG., Shiryaev SA., Golubkov VS., Ouyang M., Wang Y., and Strongin AY. (2010) A femtomol range FRET biosensor reports exceedingly low levels of cell surface furin: implications for the processing of anthrax protective antigen, PLoS ONE, 5(6):e11305 37. Qian T., Wang Y. (2010) Micro/nano-fabrication Technologies for Cell Biology. Medical & Biological Engineering & Computing, 48(10):1023-32 36. Liu B., Kim T., Wang Y. (2010) Live Cell Imaging of Mechanotransduction. Journal of the Royal Society Interface, 7 Suppl 3:S365-75 35. Ouyang M.*, Huang H.*, Shaner N., Remacle AG., Shiryaev SA., Strongin AY., Tsien RY., Wang Y. (2010) Simultaneous Visualization of Pro-tumorigenic Src and MT1-MMP Activities with Fluorescence Resonance Energy Transfer. Cancer Research, 70(6):2204-12 2009 34. Poh Y.C., Na S., Chowdhury F., Ouyang M., Wang Y., Wang N. (2009) Rapid Activation of Rac GTPase in Living Cells by Force Is Independent of Src. PLoS ONE,4(11):e7886 33. Wang Y., Wang N. (2009) FRET and Mechanobiology. Integrative Biology, 1(10):565-73 (Top Ten Article) 32. Wang Y.*, Flores L.*, Lu S., Miao H., Li Y.-S., Chien S. (2009) Shear Stress Regulates the Flk-1/Cbl/PI3K/NF-kB Pathway via Actin and Tyrosine Kinases. Cellular and Molecular Bioengineering, 2(3):341-350 31. Kim T., Wang Y. (2009) Single cell imaging of calcium dynamics in response to mechanical stimulation. Cell Mechanics: From Single Scale-Based Models to MultiscaleModeling, 10:265-279 30. Lu S., Wang Y. (2009) Application of FRET Biosensors and Computational Analysis for Live Cell Imaging. Proc. of SPIE. 7191 (719108):1-12 29. Kim T., Xu J., Dong R., Lu S., Nuzzo R., Wang Y. (2009) Visualizing the Effect of Microenvironment on the Spatiotemporal RhoA and Src Activities in Live Cells by FRET. Small, 5(12):1453-9. 28. Na S., Chowdhury F., Tay B., Ouyang M., Gregor M., Wang Y., Wiche G., Wang N. (2009) Plectin contributes to mechanical properties of living cells. Am J PhysiolCell Physiol, 296(4):C868-77 27. Seong J., Lu, S., Ouyang M., Huang H., Zhang J., Frame MC., Wang Y. (2009) Visualization of Src activity at different compartments of the plasma membrane by FRET imaging. Chemistry & Biology (Cell Press), 16(1):48-57, (Cover Article) 26. Kim T., Seong J., Ouyang M., Sun, J., Lu, S., Hong J., Wang, N., Wang Y. (2009) Mechanical rigidity regulates Ca2+ oscillation via RhoA signaling pathway in humanmesenchymal stem cells. Journal of Cellular Physiology, 218(2):285-93 2008 25. *Eichorst J., *Lu S., Xu J., Wang, Y. (2008) Differential RhoA Dynamics in Migratory and Stationary Cells Measured by FRET and Automated Image Analysis. PLoS ONE, 3(12):e4082 24. Ouyang M., Sun J., Chien S., Wang Y. (2008) Determination of hierarchical relationship of Src and Rac at subcellular locations with FRET biosensors, Proc Natl Acad SciUSA. 105(38):14353-8 23. Wang, Y., Shyy Y.-J., Chien, S. (2008) Fluorescence Proteins, Live Cell Imaging, and Mechanobiology: Seeing is Believing, Annual Review of Biomedical Engineering, 10:1-38 (The most downloaded article of ARBE in year 2009) 22. Lu S., Ouyang M., Seong J., Zhang J., Chien, S., Wang Y. (2008) The Spatiotemporal Pattern of Src Activation at Lipid Rafts Revealed by Diffusion-Corrected FRET Imaging. PLoS Computational Biology, 4(7): e1000127, (Featured Research by PLoS Computational Biology) 21. Na S., Collin O., Chowdery F., Tay B., Ouyang M., Wang Y., Wang N. (2008) Rapid signal transduction in living cells is a unique feature of mechanotransduction. ProcNatl Acad Sci USA. 105 (18):6626-31 20. Ouyang M., Lu S., Li X.-Y., Xu J., Seong J., Giepmans B., Shyy Y.-J., Weiss SJ, Wang Y. (2008) Directional MT1-MMP Activity in Live Cells Visualized by a FRET Biosensor, Journal of Biological Chemistry. 283 (25):17740-8 19. Wang Y., Lu S. (2008) The Application of FRET Biosensors to Visualize Src Activation. Proc. of SPIE. 6868 (68680A):1-9 18. Wang Y., Shyy, J.-Y., and Chien, S., (2008) “Fluorescence Live-Cell Imaging: Principles and Applications in Mechanobiology”. Bioengineering in Cell and Tissue Research, pp. 65-84 2007 17. Wang Y. and Chien, S., (2007) “Analysis of Integrin Signaling by Fluorescence Resonance Energy Transfer”. Methods in Enzymology, 426:177-201 16. Botvinick, EL and Wang Y., (2007) “Laser Tweezers in the Study of Mechanobiology in Live Cells”. Methods in Cell Biology, 82:497-523 15. Wang Y., Chang JM., Chen K.-D., Li S., Li J., Wu C., Chien S. (2007) Selective Adapter Recruitment and Differential Signaling Networks by VEGF vs. Shear Stress.Proc Natl Acad Sci USA. 104 (21): 8875-9 14. Larina IV., Wang Y., Chien S., Lane ME., and Dickinson ME. (2007) On the way to subcellular imaging of mechanotransduction in the developing vasculature. Proc. of SPIE. 6535 (653504):1-6 2006 13. Hu YL., Haga JH., Miao H., Wang Y., Li YS., Chien S. (2006) Roles of microfilaments and microtubules in paxillin dynamics. Biochem Biophys Res Commun.348(4):1463-71. 12. Wang Y., Jin G., Miao H., Li J., Usami S., Chien S. (2006) Integrins Regulate VE-cadherin and Catenins: Dependence of This Regulation on Src, but not on Ras. ProcNatl Acad Sci USA. 103(6):1774-79. 1998-2005 11. Wang Y., Botvinick E., Zhao Y., Berns M., Usami S., Tsien R.Y., Chien S. (2005) Visualization of Mechanical Activation of Src. Nature. 434:1040. 10. Miao H., Hu Y., Shiu Y.-T., Yuan S., Zhao Y., Kaunas R., Wang Y., Jin G., Usami S., and Chien S. (2005) Effects of Flow Patterns on the Localization and Expression of VE-Cadherin at Vascular Endothelial Cell Junctions: In-vivo and In-vitro Investigations. Journal of Vascular Research. 42:77. 9. Wang Y., Chang JM., Li Y.-C., Li Y.-S., Shyy J. Y.-J., and Chien S. (2004) Shear Stress and VEGF Activate IKK via Flk-1/Cbl/Akt Signaling Pathway. Am J PhysiolHeart Circ Physiol. 286(2):H685-92. 8. Shiu Y.-T., Li S., Yuan S., Wang Y., Nguyen P., Chien S. (2003) Shear stress-induced c-fos activation is mediated by Rho in a calcium-dependent manner. BiochemBiophys Res Commun. 303(2):548-55 7. Wang Y., Miao H., Li S., Chen K.-D., Li Y.-S., Yuan S., Shyy J. Y.-J., and Chien S., (2002) Interplay between integrins and FLK-1 in shear stress- induced signaling. Am J Physiol Cell Physiol. 283: C1540-1548 6. Miao H., Yuan S., Wang Y., Tsygankov A., Chien S. (2002) Role of Cbl in Shear-Activation of PI 3-kinase and JNK in Endothelial Cells. Biochem Biophys ResCommun. 292(4):892-9 5. Li S., Butler P., Wang Y., Hu Y., Han D., Usami S., Guan J.-L., Chien S. (2002). The role of the dynamic of focal adhesion kinase in the mechanotaxis of endothelial cells.Proc Natl Acad Sci USA 99(6):3546-51 4. Wu W., Wang Y., Yan Z., Wen G. (1999). The Dynamic Coupling Model for the Whole Circulation in Human Body. Acta Scientiarum Naturalium, UniversitatisPekinensis 35(3): 328-339 3. Wu W., Wen G., Yan Z., Wang Y., Zhang, Z. (1999). The Dynamic Coupling of Right Ventricle and Pulmonary Arteries. Acta Scientiarum Naturalium, UniversitatisPekinensis 35(3): 317-327 2. Wang Y., Wu W., Wen G. (1999). The Influence of Aortic Valve on the Cardiovascular Coupling. Journal of Hydrodynamics 14(1): 13-23. 1. Wang Y., Wu W., Wen G. (1998). The Influence of Cardiovascular Dynamic Coupling on the Blood Pulse Wave Propagation in Human Body. Applied Mathematics and Mechanics 19(7):601-614
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